cores: The High Throughput Screening Center for Neurogenetics
BSi Functional Genomics Service Form

   

minli@jhmi.edu

Part III. Robotic dispensing service

1. Format of the substrate plates of shRNA constructs or clones

       

2. Coverage of the shRNA constructs or clones

   

3. Amount of the shRNA constructs or clones

       

Part IV. Full service of high throughput screens:

A: Scheme of the assay principle

Specifics:

1. Assay type

2. Ever tried in multi-well microplate

   

           

3. Readout Methods

Plate-reader Methods

4. Is there "a gold standard" for the assay?

Reference

B: Assay Protocol

Is a detailed protocol available?

   

Detailed protocol includes: 1) Reagents; 2) Instruments; 3) Steps of assay.

Specifics

1.            

2.        

3. Controls

4. Automation potential

    Need filtration    Need washing    Long time incubation

5. Has the protocol been optimized?

    No

C: Data processing

Methods

3) Statistics